(227) Analysis of Gene Expression of Vitamin D in Rats in Experimental Model of Peyronie's Disease and Human Penile Tunica Albuginea

Abstract Introduction Peyronie's disease (PD) is a penile connective tissue disorder that results in an inflammatory process, fibrotic plaque the tunica albuginea (TA) and curvature. The molecular changes of formation are complex include different cellular/protein mechanisms. Vitamin D (vitD) steroid hormone has unconventional pathways action, among its effects it already been described plays role extracellular matrix remodeling, immune response fibrosis. literature regarding action vitD male genital system scarce, gene expression receptors not yet demonstrated TA. Objective To identify receptor TA rats submitted to experimental model PD human Methods divided into 2 groups (case control) before by Cohen et al. Human was achieved from Peyronie´s surgery cases cadaveric controls. antibody anti (VDR D-6: sc-13133, Santa Cruz Biotechnology®, CA, USA) used. Immunolabeling carried out using avidin-biotin-peroxidase method, 3,3′-diaminobenzidine as liquid chromogen. In each case, immunolabeling quantified computer-assisted digital image analysis following methodology Matos PCR real-time analyses followed MIQE guideline10. One fractions samples stored RNAholder. extraction total RNA reagent TRIzol®. reverse transcription performed transcriptase enzyme ImPromII™ obtain complementary DNA (cDNA). Messenger obtained RT-PCR. Quantitative RT-PCR pair oligonucleotides endogenous RPL13a (human samples) GAPDH (rat samples), known commercially forward primers. It statistical with SPSS® version 17.0 (SPSS® Inc; Illinois, USA). Values were expressed mean standard error significance level 95% (p ≤ 0.05). tests applied One-Way ANOVA unpaired t-test Welch's correction, confirming similarity between variances. Sensitivity specificity calculated Youden index. Ethical approval local committee number 06/2016. Results mRNA amplifications presence both control case groups, group statistically higher < 0,0001) when compared rats. samples, lower group. Conclusions (genic proteic expression) Peyronies´ models allows future studies regulation modulation according evolution phase serum levels. Disclosure No